Cancer Cell Line Encyclopedia Metabolomics

2012 the cancer cell line encyclopedia enables predictive modelling of anticancer drug sensitivity.

Cancer cell line encyclopedia metabolomics. To further understand the metabolic diversity of cancer we profiled 225 metabolites in 928 cell lines from more than 20 cancer types in the cancer cell line encyclopedia ccle using liquid chromatography mass spectrometry lc ms. To further understand the metabolic diversity of cancer we profiled 225 metabolites in 928 cell lines from more than 20 cancer types in the cancer cell line encyclopedia ccle using liquid. Factors influencing metabolomics raw data. 2020 the broad institute of mit harvard.

Nmr based metabolomics have also been used to identify biomarkers for cancer including hepatocellular carcinoma hcc colon cancer ovarian cancer breast cancer prostate cancer colorectal cancer and others 4 35 36 37 38 39 40 41. The ccle cancer cell line encyclopedia project is a collaboration between the broad institute and the novartis institutes for biomedical research and its genomics institute of the novartis research foundation to conduct a detailed genetic and pharmacologic characterization of a large panel of human cancer models to develop integrated computational analyses that link distinct pharmacologic vulnerabilities to genomic patterns and to translate cell line integrative genomics into cancer. When coupled with pharmacological profiles for 24 anticancer drugs across 479 of the cell lines this collection allowed identification of genetic lineage and gene expression based predictors of drug sensitivity. The experiment was performed in multiplex format with 9 biological samples per plex and one common sample to normalize between plexes.

The cancer cell line encyclopedia is a database of gene expression genotype and drug sensitivity data for human cancer cell lines. A compilation of gene expression chromosomal copy number and massively parallel sequencing data from 947 human cancer cell lines. Citation s barretina j et al. In a companion paper in nature medicine another team led by sellers chemical biology and therapeutics science program graduate student haoxin li and institute scientist and metabolomics platform senior director clary clish also opened a new view into cancer biology by probing the abundances of 225 metabolites of 928 of the ccle lines the first such systematic metabolomic survey of a cell line collection of this size and diversity.